Nippon Genetics FastGene Scriptase III cDNA 5x ReadyMix incl. Oligo-dTs and Random Hexamers - 100 Reactions

The FastGene® Scriptase III is an enhanced variant of the Murine Leukemia Virus (MuLV) reverse transcriptase. Due to specific mutations in the RNase H domain of the reverse transcriptase, cDNA can be synthesized even from very small amounts of RNA.

Scriptase III has additional mutations that make it even more robust and thermostable. It can therefore operate with maximum activity up to a temperature of 55°C. At these elevated temperatures, highly structured RNAs can easily unfold, allowing them to serve as templates for reverse transcription. It is the superior choice for complex tasks, such as Real-Time PCR and NGS.

• Simplify your reverse transcription workflow with the FastGene® Scriptase III Ready Mix, a 5x master mix that’s ready to use right out of the box
• All-in-one solution combines Scriptase III, optimised buffer, dNTPs, and an RNase inhibitor, ensuring robust and efficient cDNA synthesis
• Kit also includes separate tubes with random primers and oligo(dT) primers, making it versatile for both prokaryotic and eukaryotic RNA samples
• FastGene Scriptase III cDNA 5x ReadyMix components:
• Scriptase III (200 units/µL)
• 10X RT buffer
• dNTP mixture (10mM each)
• RNase inhibitor
• Separate tubes:
• Oligo dT (80µM)
• Random hexamer (50ng/µL)